SpeciesReactivity
Mouse

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Anti-Mouse IgG coated strip plate (8 wells x 12 strips) 6321 2. Mouse IgG Std. A (0 ng/ml), 0.25 ml 6322 3. Mouse IgG Std. B (50 ng/ml), 0. 25 ml 6323 4. Mouse IgG Std. C (100 ng/ml), 0. 25 ml 6324 5. Mouse IgG Std. D (300 ng/ml), 0. 25 ml 6325 6. Mouse IgG Std. E (600 ng/ml), 0. 25 ml 6326 7. Mouse IgG Std. F (1000 ng/ml), 0. 25 ml 6327 8. 20x Sample Diluent, 10ml SD-20 9. Wash Buffer (100X), 10 ml WB-100 10. HRP conjugate diluent, 12ml HD-1 11. 100x Anti-mouse IgG-HRP Conjugate, 0.12 ml 6328 12. TMB Substrate, 12 ml 80091 13. Stop solution, 12 ml 80101 14. Instruction Manual M-6320

SpCrossreactivity
The antibodies used in this kit are not specific for any IgG subtype (IgG1, IgG2, etc.). This kit is also specific to IgG and does not show any significant reactivity to mouse IgA, IgM, IgE or other serum proteins. Mouse IgG ELISA kit species crossreactivity was tested with the following animal serum at dilutions of 1:4k: Significant crossreactivity was observed with rat (16%), and hamster (3%) serum. Guinea Pig, Rabbit, FBS, Bovine, Goat, Sheep, Pig, Human, Monkey, and Chicken serum IgG had less than 1% crossreactivity.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.